Journal: PLoS ONE

Article Title: The “ Artificial Artery ” as In Vitro Perfusion Model

doi: 10.1371/journal.pone.0057227

Figure Lengend Snippet: ( A ) Longitudinal section of the inside of a hollow fiber membrane confluently colonized with HUVECs and stained with Hoechst 33342 after 0.1 N/m 2 applied for 24 h (magnification: 1∶100). ( B ) Longitudinal section of the outside of a HUASMC mono-culture module stimulated with low laminar FSS for 24 h (magnification: 1∶100). ( C ) Cross-section of a polypropylene hollow fiber co-colonized with HUVECs and HUASMCs. In focus are HUVECs on the inside upon 0.1 N/m 2 applied for five days (magnification: 1∶100). ( D ) Cross-section of a co-culture module showing HUASMCs on the outside of a hollow fiber after low laminar FSS stimulation for five days (magnification: 1∶100).

Article Snippet: Cryopreserved human umbilical vein endothelial cells (HUVECs) and cryopreserved human umbilical artery smooth muscle cells (HUASMCs) were purchased from PromoCell (C-12203, C-22050).

Techniques: Staining, Co-Culture Assay

Journal: PLoS ONE

Article Title: The “ Artificial Artery ” as In Vitro Perfusion Model

doi: 10.1371/journal.pone.0057227

Figure Lengend Snippet: ( A ) Scanning electron microscopy picture of the homogenously colonized inside of a hollow fiber with confluently grown and characteristically cobblestone shaped human primary endothelial cells upon the application of low laminar FSS (0.1 N/m 2 ) for 24 h (magnification: 1∶500). ( B ) Scanning electron microscopy picture of HUASMCs on the hollow fiber outside with their typical cell cytoskeletal structure and morphology upon 0.1 N/m 2 applied for 24 h (magnification: 1∶400). ( C ) Confocal microscopic immunolocalization of Cadherin-5 in co-cultivated HUVECs exposed to low laminar FSS (0.1 N/m 2 ) over a period of five days (magnification: 1∶400). ( D )Cadherin-5 in HUVECs upon high laminar FSS (3 N/m 2 ) (magnification: 1∶400). ( E ) Confocal microscopic immunolocalization of α-smooth-muscle-actin in co-cultivated HUASMCs upon 3 N/m 2 luminally applied for a five day period (magnification: 1∶400). ( F ) α-smooth-muscle-actin in co-cultivated HUASMCs upon high laminar FSS (3 N/m 2 ) (magnification: 1∶400).

Article Snippet: Cryopreserved human umbilical vein endothelial cells (HUVECs) and cryopreserved human umbilical artery smooth muscle cells (HUASMCs) were purchased from PromoCell (C-12203, C-22050).

Techniques: Electron Microscopy

Journal: PLoS ONE

Article Title: The “ Artificial Artery ” as In Vitro Perfusion Model

doi: 10.1371/journal.pone.0057227

Figure Lengend Snippet: Semi-quantitative RT-PCR analysis showing the total VWF mRNA expression in co-colonized HUVECs and HUASMCs. RNA of both cell types was isolated separately. The expression of VWF in 0.1 N/m 2 stimulated HUVECs was taken as reference. ( A ) MALDI mass spectrum from supernatants of the “ artificial artery” after each day. Randomly selected peptides show constant molecular mass-signal intensities over a period of five days (abscissa: relative molecular mass m/z, z = 1; ordinate: relative intensity, arbitrary units). ( B ) MALDI mass spectrum of the supernatant of endothelial cells after stimulation with 3.0 N/m 2 (upper spectrum) and without stimulation (0.1 N/m 2 ) (lower spectrum) (abscissa: relative molecular mass, m/z, z = 1; ordinate: relative intensity, arbitrary units). ( C ) Relative mass-signal intensities of Up 4 A in secretomes isolated from HUVECs, HUASMCs, and HUVEC/HUASMC co-cultures in the “ artificial artery ” after stimulating with 3 N/m 2 for five days. ( D ) Semi-quantitative RT-PCR analyses showing the total mRNA expression of KLF2, TIMP1, and CCND1 in co-colonized HUVECs exposed to 3 N/m 2 for five days. The expression of each gene in 0.1 N/m 2 stimulated HUVECs was taken as reference. ( E ) Semi-quantitative RT-PCR analysis showing the total EDN1 mRNA expression in co-colonized HUVECs (0.1 N/m 2 vs. 3 N/m 2 ) and HUASMCs (0.1 N/m 2 vs. 3 N/m 2 ). The expression of END1 in 0.1 N/m 2 stimulated HUVECs was taken as reference.

Article Snippet: Cryopreserved human umbilical vein endothelial cells (HUVECs) and cryopreserved human umbilical artery smooth muscle cells (HUASMCs) were purchased from PromoCell (C-12203, C-22050).

Techniques: Quantitative RT-PCR, Expressing, Isolation